Journal: CNS Neuroscience & Therapeutics

Article Title: The Role of P62 /Nrf2/Keap1 Signaling Pathway in Lead‐Induced Neurological Dysfunction

doi: 10.1111/cns.70566

Figure Lengend Snippet: Disruption of the Keap1/Nrf2 signaling pathway mediates Pb‐induced cellular oxidative damage. (A) Immunofluorescence detection of Nrf2 expression in Pb‐exposed SH‐SYS5Y cells; Green: Nrf2; Blue: DAPI. (B) Detection of Nrf2, Keap1, and HO‐1 proteins by Western blot in Pb‐exposed SH‐SYS5Y cells. (C) Detection of Nrf2, Keap1, and HO‐1 protein expression in the hippocampus of Pb‐exposed rats. Quantitative data was presented as mean ± SD ( n = 3) and analyzed by one‐way ANOVA or Student's t ‐test.

Article Snippet: To activate Nrf2, SH‐SY5Y cells exposed to Pb were pretreated with the Nrf2 activator Artemisitene (ATT) (MedChemExpress, MCE, HY‐122550) [ ].

Techniques: Disruption, Immunofluorescence, Expressing, Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: The Role of P62 /Nrf2/Keap1 Signaling Pathway in Lead‐Induced Neurological Dysfunction

doi: 10.1111/cns.70566

Figure Lengend Snippet: P62 crosstalks with the Nrf2/Keap1 axis in the hippocampus of Pb‐exposed rats. Immunofluorescence detection of Nrf2, P62, and Keap1 expression levels and co‐localization in the hippocampus of Pb‐exposed rats. Green: Nrf2; Red: P62; Blue: DAPI; Purple: Keap1.

Article Snippet: To activate Nrf2, SH‐SY5Y cells exposed to Pb were pretreated with the Nrf2 activator Artemisitene (ATT) (MedChemExpress, MCE, HY‐122550) [ ].

Techniques: Immunofluorescence, Expressing

Journal: CNS Neuroscience & Therapeutics

Article Title: The Role of P62 /Nrf2/Keap1 Signaling Pathway in Lead‐Induced Neurological Dysfunction

doi: 10.1111/cns.70566

Figure Lengend Snippet: Inhibition oxidative stress alleviates Pb‐induced neurodegeneration via improving the P62/Nrf2/Keap1 pathway. SH‐SY5Y cells were pre‐treated with NAC for 1 h followed by exposure to 50 μM Pb for 24 h. (A) DCFH‐DA probe was used to detect ROS levels; Green: ROS. (B) Western blot analysis was performed to determine Nrf2, Keap1, and HO‐1 protein expressions. (C) Western blot analysis was conducted to assess LC3, P62, and Tau protein expressions in the hippocampus of Pb‐exposed rats. Quantitative data was presented as mean ± SD ( n = 3) and analyzed by one‐way ANOVA.

Article Snippet: To activate Nrf2, SH‐SY5Y cells exposed to Pb were pretreated with the Nrf2 activator Artemisitene (ATT) (MedChemExpress, MCE, HY‐122550) [ ].

Techniques: Inhibition, Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: The Role of P62 /Nrf2/Keap1 Signaling Pathway in Lead‐Induced Neurological Dysfunction

doi: 10.1111/cns.70566

Figure Lengend Snippet: Nrf2 activator Artemisitene alleviate Pb‐induced neurological dysfunction. SH‐SY5Y cells were pre‐treated with 2 μM Artemisitene (ATT) for 1 h followed by exposure to 50 μM Pb for 24 h. (A) Detection of Nrf2, Keap1, and HO‐1 proteins by Western blot. (B) Detection of P62, LC3, P62, P‐Tau and total Tau proteins by Western blot. Quantitative data was presented as mean ± SD ( n = 3) and analyzed by one‐way ANOVA.

Article Snippet: To activate Nrf2, SH‐SY5Y cells exposed to Pb were pretreated with the Nrf2 activator Artemisitene (ATT) (MedChemExpress, MCE, HY‐122550) [ ].

Techniques: Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: The Role of P62 /Nrf2/Keap1 Signaling Pathway in Lead‐Induced Neurological Dysfunction

doi: 10.1111/cns.70566

Figure Lengend Snippet: Rapamycin alleviates lead‐induced neurological dysfunction by promoting autophagy. SH‐SY5Y cells were pre‐treated with 1 μM Rapamycin (RAPA) for 4 h followed by exposure to 50 μM Pb for 24 h. (A) Detection of Nrf2, Keap1, and HO‐1 proteins by Western blot. (B) Detection of P62, LC3, P‐Tau and total Tau proteins by Western blot. Quantitative data was presented as mean ± SD ( n = 3) and analyzed by one‐way ANOVA.

Article Snippet: To activate Nrf2, SH‐SY5Y cells exposed to Pb were pretreated with the Nrf2 activator Artemisitene (ATT) (MedChemExpress, MCE, HY‐122550) [ ].

Techniques: Western Blot